Journal: In Vitro Cellular & Developmental Biology. Animal
Article Title: Correlation of molecular and cellular signatures in primary skeletal muscle satellite cells derived from lean and diet-induced obese mice
doi: 10.1007/s11626-025-01130-2
Figure Lengend Snippet: Myotube formation of satellite cell–derived myoblasts of CTL and DIO ( A ) with Western blot analysis of myogenic markers ( B – E ). ( A ) Brightfield images of differentiating myoblasts of CTL ( upper line ) and DIO ( lower line ) after 3 and 6 d of serum withdrawal. Magnified myotubes in upper right rectangles. Scale bar = 500 µm. ( B ) Western blot analysis of Myf5 protein in CTL and DIO at 3 and 6 d of proliferation or differentiation, respectively. ( C ) Western blot analysis of MyoD protein in CTL and DIO at 3 and 6 d of proliferation or differentiation, respectively. ( D ) Western blot analysis of myogenin protein in CTL and DIO at 3 and 6 d of proliferation or differentiation, respectively. ( E ) Western blot analysis of Mf20 protein in CTL and DIO at 3 and 6 d of proliferation or differentiation, respectively. ( F ) Western blot analysis of Itga7 protein in CTL and DIO satellite cell–derived myoblasts at 3 and 6 d of proliferation or differentiation, respectively. ( G ) Western blot analysis of CD56 protein in CTL and DIO at 3 and 6 d of proliferation or differentiation, respectively. ( H ) Western blot analysis of Pax7 protein in CTL and DIO at 3 and 6 d of proliferation or differentiation, respectively. Fold change of normalized samples described as a quotient of each sample with standard control (proliferation 3 d).
Article Snippet: Integrin α7-PE-Vio770 (Itga7) , Miltenyi Biotec, Bergisch Gladbach, Germany; 130-120−812 , Mouse, monoclonal , MACS, FACS.
Techniques: Derivative Assay, Western Blot, Control